Development of an EBV-Mammay Epithelial Cell Model for Addressing the Role of EBV in Breast Cancer
Abstract
In this grant, we proposed to generate an EBV infected mammary epithelial system that could be used to characterize the life cycle and oncogenic properties of EBV in this unique tissue. We have been utilizing primary human mammary epithelial cell lines generated in Myles Brown's laboratory. By Fluorescence Activated cell Sorting (FACS), we determined that these cells are positive for the EBV receptor, CR2 and we have been carrying out infection studies to determine the infectability of these cells to EBV. To date, we have found that despite the presence of the CR2 receptor, infectability is relatively low. This makes transient studies more difficult. We have therefore, begun exploring additional methods for obtaining high infection efficiencies. We believe that we are making encouraging progress which has been facilitated by the funding of this application. We are confident that our studies will lead to the development of important reagents and ultimately further funding in this area of research.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2003
- Accession Number
- ADA420086
Entities
People
- Erik K Flemington
Organizations
- Tulane University of Louisiana