Biological Function of BRCA1 and Its Regulation by Extracellular Matrix and PTEN in Breast Cancer
Abstract
Inherited mutations in the breast and ovarian cancer susceptibility gene BRCAl are associated with a high risk for developing breast and ovarian cancers. while numerous studies link BRCAl to transcriptional regulation, DNA repair, apoptosis and growth/tumor suppression, the overexpression of the ErbB-2 family of receptor tyronsine kinases has been linked to the development of non-familial or sporadic breast cancer. Activation of HRG signaling, mediated by the binding of HRG to ErbB receptors, has been implicated in the development of aggressive phenotype in breast cancer cells. The mechanisms through which HRC regulates the progression of breast cancer cells to a more invasive or motile phenotype are currently unknown. Our specific aims are: 1) To determine the effect of ECM/integrins on BRCAl expression, phosphorylation and nuclear translocation. 2) To generate inducible stable transfected T47D clones that overexpress BRCAl protein, in order to characterize its biological functions in breast cancer cells. 3) To determine the effect of PTEN on BRCAl phosphorylation.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2003
- Accession Number
- ADA420089
Entities
People
- Hava Avraham
- Tohomir Miralem
Organizations
- Beth Israel Deaconess Medical Center