Control of Metastasis-Associated Gene Expression by Cell-Surface Beta-1,6 Branched Oligosaccharide Levels
Abstract
Metastatic breast cancer cells expresss cell-surface Beta-1,6 branched oligosaccharide structures, which are reduced/absent in normal breast tissue. The biosynthesis of these structures ininitiated by the Golgi-localized glycosyltransferase N- Acetylglucosmaminyl-transferase V (GlcNAc-T V). The altered expression of GlcNAc-T V in animal mammary cancer models also influences metastasis. Furthermore, altered transcription of metastasis-associated genes has also been observed incells with a reduced expression of beta-1,6 branched oligosaccharide expression. The aim of this project is to characterize the altered expression of mRNA in mouse and human mammary cancer-derived cell lines as a function of altered Beta-1,6 branched oligosaccharide expression. This will be accomplished by using DNA Microarray technology to assess the mRNA levels in cell lines transfected with GlcNAc-T V expression vectors. After identification of mRNA molecules that are altered by changes (elevations or reduction) in cell-surface Beta-1,6 branched oligosaccharide expression, the kinetics of induction will be characterized using cell lines with GlcNAc-T V under the control of an inducible promoter.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2003
- Accession Number
- ADA420126
Entities
People
- William G. Chaney
Organizations
- University of Nebraska Medical Center