Identification of Novel Genes Affected by Gamma Irradiation Using a Gene-Trapped Library of Human Mammary Epithelial Cells

Abstract

We propose that the expression of several unknown genes is affected by gamma radiation. Abnormal expression of these genes maybe one of the early steps in breast carcinogenesis induces by radiation. We plan to establish an assay that will allow us to screen for breast cells that contain a single mutation by gene trapping. We will be able to detect changes in the expression of a gene upon treatment with different doses of radiation. These radiation-responsive genes will be identified through the rapid amplification of cDNA end procedure and sequenced. Cells that are affected by radiation will be isolated and analyzed to see if the changes can lead to transformation of the normal breast epithelial cell into a neoplastic cell. This assay may prove to be a powerful tool in the identification of novel genes that are affected by gamma irradiation in the early stages of breast cancer progression.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 2003
Accession Number
ADA420152

Entities

People

  • Jennifer Malone
  • Robert Ullrich

Organizations

  • Colorado State University

Tags

DTIC Thesaurus Topics

  • Androgen Receptors
  • Androgens
  • Breast Cancer
  • Cells
  • Chemistry
  • Culture Media
  • Diseases And Disorders
  • Environmental Health
  • Epithelial Cells
  • Gamma Rays
  • Gene Expression
  • Health Services
  • Identification
  • Neoplasms
  • Polymerase Chain Reaction
  • Proteins
  • Radiation

Fields of Study

  • Biology

Readers

  • Molecular Genetics
  • Molecular and genetic basis of cancer.
  • Nuclear and Radiation Engineering.