Tumor-Secreted Autocrine Motility Factor AMF: causal Role in an Animal Model of Cachexia

Abstract

Purified mouse autocrine motility factor/phosphoglucose isomerase was found to cause weight loss (cachexia) after 3 days of 3x daily intraperitoneal injection, which was accompanied by significant increases in serum concentrations of the factor. This is a simpler animal model than originally proposed structure of the protein complexed with inhibitor has been soved by x-ray crystallography and published. Mutant forms of the protein have been prepared. Experiments are underway to improve the purity of the recombinant protein and to characterize the effects of the factor on both intact animals and on a mouse muscle cell line in vitro.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2003
Accession Number
ADA420242

Entities

People

  • John M. Chirgwin

Organizations

  • University of Virginia

Tags

DTIC Thesaurus Topics

  • Biological Factors
  • Body Weight
  • Bone Diseases
  • Cancer
  • Cell Line
  • Cells
  • Chemistry
  • Crystal Structure
  • Crystallography
  • Escherichia Coli
  • Metabolic Diseases
  • Molecules
  • Muscle Cells
  • Peptide Growth Factors
  • Peptides
  • Proteins
  • X Rays

Readers

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