Molecular Markers of Metastasis in Ductal Mammary Carcinoma

Abstract

Representational difference analysis method was used to isolate metastasis-specific genetic markers for breast cancers that are prone to develop metastasis. Of the 15 candidate MADS that have been isolated, 3 were found to be promising markers for breast metastasis. Screening of MADS-IX on a tumor cell line with homozygous loss of PTEN gene, revealed that MADS-IX is neither a part of PTEN gene nor localized in the homozygous loss region of chromosome 10q arm encompassing PTEN. Our Fluorescence in situ hybridization screening for MADS%S on primary tumor tissue touch preparation cells was successful. The results indicate that the MADS-IX could be used as a FISH probe for distinguishing the primary tumors that did and that did not develop metastasis.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2003
Accession Number
ADA420319

Entities

People

  • Patnala M. Archary

Organizations

  • Albert Einstein College of Medicine

Tags

DTIC Thesaurus Topics

  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Genetics
  • Health Services
  • Medical Personnel
  • Oncology
  • Proteins

Fields of Study

  • Biology

Readers

  • Molecular and Cellular Biology
  • Oncology (Cancer Research).

Technology Areas

  • Biotechnology