The Functions of BRCA2 in Homologous Recombinational Repair

Abstract

The BRCA2 breast cancer gene is a major contributor to a dominantly inherited predisposition to the disease. Recent evidence has indicated that the critical functions of BRCA2 associate with chromosome stability and with DNA repair medicated by homologous recombination. Using homologous recombination assay, we have demonstrated that disruption of the BRCA2- Rad51 interaction inhibits the HRR frequency in vivo. The results indicate that the interactions between Rad5l and BRCA2 are important for regulating HRR. The 6xHis-tagged baculoviral construct of BRC1-4, BRC5-8 and BRC1-8 fragments of bRCA2 have been made and individually expressed in insect cells. Each protein was purified using a Ni-NTA column and the identity of each protein was confirmed by Western analysis. We have used three assay systems to determine the activity of Rad5lB and Rad5lC proteins, including DNA binding, ATPase and DNA strand transfer.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2003
Accession Number
ADA420343

Entities

People

  • David J. Chen

Organizations

  • University of California, Berkeley

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Amino Acids
  • Biochemistry
  • Biological Sciences
  • Breast Cancer
  • Cell Line
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Culture Techniques
  • Frequency
  • Genetic Structures
  • Genetics
  • Identities
  • Ionizing Radiation
  • Microbiology
  • Nucleic Acids
  • Precipitation

Fields of Study

  • Biology

Readers

  • Molecular Genetics
  • Women's Health and Cancer Risk Research: African American Women and Pregnancy Outcomes.

Technology Areas

  • Biotechnology