Basis of Persistant Microenvironment Perturbation in Irradiated Human Mammary Epithelial Cells
Abstract
Our hypothesis is that the disruption of morphogenesis releases constraints on genomic integrity in preneoplastic cells. Thus we predict that colonies arising from irradiated cells will show increased genomic instability due to the lack of microenvironment control rather than as a direct result of DNA damage. In the current IDEA grant, we seek to determine whether such a correlation exists. In addition, to be in a position to manipulate the phenotype in future studies, we wish to determine the underlying basis for the irradiated phenotype. In aim 2, we will determine whether irradiated cells can communicate their phenotype in a fashion similar to the bystander effect' that occurs when unirradiated cells respond as if they were irradiated under conditions of heterogeneous radiation exposure. In aim 3, we will begin studies to test an alternative%or additional mechanism in which the phenotype is perpetuated by epigenetic modifications leading to altered gene expression. Together these studies will test whether the irradiated HMEC phenotype contributes to radiation-induced genomic instability that is observed in cells generations after radiation exposure.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2003
- Accession Number
- ADA420373
Entities
People
- Mary H. Barcellos-hoff
Organizations
- University of California, Berkeley