Growth-Promoting and Angiogenic Functions in Adenosine in Breast Cancer
Abstract
Adenosine has angiogenic, growth-stimulatory and immunosuppressive properties that may be relevant for breast cancer progression. The second period of the funding term (12 months, Aug 1, 2002 to Jul 31, 2003) has been used to characterize in detail breast cancer cell lines developed in the first period. The in-depth characterization of eN-1 GFP-, and ADA overexpressing clones of MDA-MV-231 cells included their expression profiling and adhesion properties in vitro assays. The expression profiling of the panel of breast cancer cells established that the high capacity to generate adenosine correlates with mesenchymal phenotype that is characteristic for invasive and metastatic breast cancer cells. Several membrane proteins that are characteristic for this phenotype, such as CD44, integrins alpha5 and beta1 and EGFR were not altered in GFP and ADA overexpressing and in eN-suppressed MDA-MB-231 cells, suggesting that engineerd alterations were confined to the capacity to produce adenosine and express eN. Characterization of eN and ADA+ cells led to discovery that eN is a receptor for Tenascin C and downregulation of eN caused decreased adhesion on this ECM. Finally, we have performed preliminary inoculations of developed cells in nude mice to test the relationship of growth of grafts to the expression of ADA and eN. Our results so far fully support the important role of adenosine and eN in breast cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2003
- Accession Number
- ADA420393
Entities
People
- Jozef Spychala
Organizations
- University of North Carolina at Chapel Hill