The Role of Claudin-7 in Mouse Mammary Gland and Tumorigenesis
Abstract
We have fully characterized the expression and localization of claudin 7 in the mammary epithelium, finding that it is not associated with tight junctions but is constitutively present in punctate structures, presumably vesicles, at the basal and lateral borders of cells. We find the same to be true of mouse mammary tumors. We find claudins 1, 3, 4, 5 and 8 to be present in at least one of our mouse mammary cell lines, where they are localized to the tight junction. Only claudin 1 has been found to be consistently present in mammary epithelial tight junctions. Claudin 3 is localized to the basal and lateral borders of the cells in the virgin and pregnant animals, but not observed in lactation. Claudin 8 is prominent at the tight junctions in the mammary epithelium from the lactating animal. In the next year we will complete these analyses and apply them to mouse mammary tumors. In addition we plan to use our adenoviral technology to overexpress claudin 8 during pregnancy to determine whether it can contribute to closure of the tight junctions under conditions where the junctions are normally open.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2003
- Accession Number
- ADA420404
Entities
People
- Margaret Neville
Organizations
- University of Colorado Health