The Quantitative Immunophenotype (QuIP) is Characteristic of Individuals
Abstract
The clusters of differentiation (CD) molecules used to identify lineages of cells often have important functional activities. Furthermore, the numbers of CD molecules expressed per cell can reflect the status of cell lineages. For example, B cells with abnormal numbers of CD19 per cell have altered capacities to produce antibody responses. Therefore, the quantitative immunophenotype (QuIP), i.e., both the numbers of cells in various populations and the numbers of CD molecules expressed per cell, can provide additional information about the functional capacity of the immune system. In order to examine the variations in QuIP among individuals and within individuals over time, blood samples from a cohort of Marines were tested three times over a two month period before and after various phases of strenuous advanced field training exercises. Samples were examined via a standard hematology analyzer to obtain complete blood counts and flow cytometrically with a panel of antibodies. The relative numbers of CD molecules per cell were estimated by using standard beads with known numbers of fluorochrome molecules per bead or with known antigen binding capacities per bead. Within individuals, the relative percentages of cell lineages and the numbers of CD molecules expressed per cell changed very little over two months although there was substantial variation among individuals at each time. Thus, measuring the QuIP can provide additional and potentially valuable information about the status of the immune system.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 14, 2000
- Accession Number
- ADA420497
Entities
People
- Donald E. Roberts
- Doug Redelman
- Wayne Ensign
Organizations
- Naval Health Research Center