A Modified Rapid Method of Nucleic Acid Isolation From Suspension of Matured Virus: Applied in Restriction Analysis of DNA from an Adenovirus Prototype Strain and a Patient Isolate

Abstract

This report describes a method for the isolation of nucleic acid from a suspension of matured virus. Nucleic acid (DNA) was isolated from a prototype strain of adenovirus type 7 and a clinical isolate of adenovirus type 7. Instead of the usual method of ultracentifugation, a filtration method was applied to concentrate the virus rapidly and nucleic acid was then isolated by a standard phenollchloroformlisoamyl-alcohol extraction procedure. The DNA was found to be sufficiently purified to generate a reproducible restriction endonuclease digestion pattern. The clinical isolate of adenovirus type 7 revealed loss of restriction site for the endonuclease HindIll when compared with the prototype strain.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2001
Accession Number
ADA420675

Entities

People

  • Charles T. Le
  • Gregory C. Gray
  • Saibol K. Poddar

Organizations

  • Naval Health Research Center

Tags

DTIC Thesaurus Topics

  • Amplification
  • Biology
  • Chloroform
  • Culture Techniques
  • Department Of Defense
  • Detection
  • Digestive System Processes
  • Diseases And Disorders
  • Environmental Health
  • Extraction
  • Filtration
  • Medical Personnel
  • Nucleic Acids
  • Phosphodiesterases
  • Standards
  • Virology
  • Viruses

Fields of Study

  • Biology
  • Computer science

Readers

  • Molecular Genetics
  • Systems Analysis and Design

Technology Areas

  • Biotechnology