Mechanisms of Intraductal Tumor Spread
Abstract
During the administrative funding period of this grant, we have developed a system that permits three-dimensional reconstruction of intraductal tumors (DCIS) from physical tissue sections. The system reduces the interaction required for low-resolution imaging of H&E stained sections, registration of images of consecutive sections and annotation of tissue structures -i.e. morphologically normal ducts and intraductal tumors-in the images. In addition, we have developed fully automatic tools for image registration and annotation that are now being integrated in our system and used in the reconstruction of the latest tissue specimens. Complementing morphological H&E based reconstructon, our system can be used for morphologically driven acquisition of high-resolution images from immunostained intermediate sections, both using fluorescence and brightfield microscopy. We are using our system to characterize the cellular differences between malignant transformed cells and morphologically normal cells of ducts either in continuum or in the proximity of DCIS tumors. Under the no-cost extension requested, we expect to complete the characterization, which includes the following markers: Her2, ER and PR status Ki67 (proliferation), Caspase 3 (apoptosis) and a marker of dedifferentiation of stemness, CD49f.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2003
- Accession Number
- ADA420771
Entities
People
- Carlos O. De Solorzano
Organizations
- University of California, Berkeley