Isolation of Estrogen-Responsive Genes in Human Breast Cancer Cells

Abstract

The goal of this research is to isolate and identi% the estrogen receptor (ER) associated genes in human breast cancer cells. Approximately 60% of ER positive human breast tumors need estrogen to grow, and the need for estrogen corresponds to the presence of the estrogen receptor in the tumor. ER is a transcription factor that binds directly or indirectly to regulatory regions of estrogen responsive genes. In the presence of estradiol (E2), ER dimerizes and binds to estrogen response elements (ERE) to turn on or turn off the expression of the genes, which are required in order for breast cancer cells to proliferate (Luqmani et al., 1989; Berry et al., 1989). A number of estrogen-inducible genes, such as cathepsin D and c-fos genes, have ER binding to DNA with help from another transcription factor Spi (Krishnan et al., 1994; Safe, 2001; Duan et al., 1998). In some cases, ER operates through Spi without binding to DNA (xie et al., 1999; Duan et al., 1998; Dubik and Shiu, 1992).

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2003
Accession Number
ADA420776

Entities

People

  • James R. Davis

Organizations

  • University of Manitoba

Tags

DTIC Thesaurus Topics

  • Birds
  • Breast Cancer
  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Enzyme Inhibitors
  • Gene Expression
  • Genetic Structures
  • Medical Personnel
  • Neoplasms
  • Nutritional Sciences
  • Organic Chemistry

Fields of Study

  • Biology

Readers

  • Breast cancer cell signaling and growth regulation.
  • Molecular Genetics