Breast Cancer Risk in Relation to Urinary Estrogen Metabolites and Their Genetic Determinants: A Study Within the Dutch "DOM" Cohort
Abstract
We are conducting a large case-control study, nested within a prospective cohort, to estimate relative risk of breast cancer in post-menopausal women by levels of urinary estrogens and estrogen metabolites, and to examine the association between the concentrations of these hormones and breast cancer risk with polymorphic variations of a series of candidate genes, known to be implicated in the regulation of estrogen metabolites synthesis. In this first year of the project, cases and matched control subjects were identified, and their urinary samples retrieved from the biorepository in Utrecht (the Netherlands). After centrifugation of the urine samples to prepare the pellets for DNA analysis, cases and matched controls were sent to Dr. Kurzer's laboratory (University of Minnesota, USA) for hormonal analyses by gas chromatography/mass-spectrometry. The laboratory of Dr. Kurzer had been prepared, and her personnel hired for running these analyses. Our study started without problems, and is mostly on schedule. Urinary DNA has been extracted and prepared for genotyping of polymorphic variants in CYP1A1, CYP1B1, CYP3A4 and COMT genes, which will be measured in year 3 of the project. Measurements of estrogen metabolites will start in September/October 2003.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2003
- Accession Number
- ADA420792
Entities
People
- Rudolf J. Kaaks
Organizations
- International Agency for Research on Cancer