USF-2 as an Inhibitor of Mammary Gland Carcinogenesis
Abstract
The hypothesis tested in these studies is that overexpression of USF in the mammary glands of transgenic mice will inhibit myc-dependent tumorigenesis. Expression of a FLAG-tagged form or USF-2 was targeted to the mammary gland under the control of the mouse mammary tumor virus (mmtv) long terminal repeat. Of eight lines of transgenic mice that were generated, one demonstrated expression of flag-tagged USF-2 in the lactating mammary gland at levels 12-fold over that of endogenous USF-2. Evaluation of tumorigenesis in these mice (n=17) out to 453 days of age suggests that USF-2 when overexpressed by itself is not oncogenic. In contrast, 72% of mmtv-myc mice (n=18) analyzed have developed mammary tumors with an average latency of 156+/-33 days. Tumor frequency and latency in bigenic mmtv-USF-2/mmtv-C-myc mice out to 200 days of age was similar to that in mmtv-C-myc mice. In contrast the growth of these myc-dependent tumors in bigenic mice was significantly lower (P<O.O1) than that in mmtv-C-myc mice. This decreased growth, however was not associated with changes in cell proliferation or death. These data support the conclusion that while overexpression of USF-2 has only modest impact on mammary gland development and myc-dependent tumorigenesis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2003
- Accession Number
- ADA420853
Entities
People
- Darryl L. Hadsell
Organizations
- Baylor College of Medicine