Tc-99m Labeled and VIP Receptor Targeted Liposomes for Effective Imaging of Breast Cancer
Abstract
Receptors for vasoactive intestinal peptide (VIP-R) are overexpressed in human breast cancer. This phenomenon may have important diagnostic and therapeutic implications because carrier systems such as sterically stabilized liposomes (SSL) loaded with imaging or therapeutic agents, and with surface ligands specific to VIP-R could potentially be actively targeted to breast cancer. This part of the project aims to test the targeting ability of VIP-SSL to n-methyl nitrosourea (MNU)-induced rat breast cancer in vitro. First, VIP was conjugated to an activated DSPE-PEG (DSPE-PEG-NHS) under mild conditions to obtain a predominantly 1:1 conjugate of VIP and DSPE-PEG (DSPE-PEG-VIP), as evidenced by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). To test breast cancer targeting ability in vitro, DSPE-PEG-VIP was inserted into preformed fluorescent cholesterol (BODIPY-Chol) labeled SSL by incubation at 37 deg C. These VIP-SSL were incubated with MNU-induced rat breast cancer tissue sections. The results showed that when compared to fluorescent SSL without VIP or non-covalently attached VIP, significantly more VIP-SSL were attached to rat breast cancer tissues indicating that SSL with covalently attached VIP can be actively targeted to rat breast cancer tissues. This targeted carrier system is currently being explored for functional imaging of breast cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2003
- Accession Number
- ADA421132
Entities
People
- Hayat Önyüksel
Organizations
- University of Illinois at Chicago