Y Chromosome Aberrations in Prostate Cancer
Abstract
We hypothesize that chromosomal loss of the Y chromosome in prostate cancer is a specific tumorigenic event. Transfer of the Y chromosome by microcell mediated chromosome transfer into PC-3 cells resulted in suppression of tumor growth. However, these PC-3 hybrids containing the Y chromosome did grow in soft agar indicating a lack of correlation between in vitro and in vivo tumor growth. We have isolated clones with fragments of the Y chromosome and found suppression of tumorigenesis correlates with either the short arm of the Y or two small regions on the q arm. The data from the functional assay will be correlated with data form deletions in patient material. We have isolated a complete genomic microarray specific for the Y chromosome. These BAC (bacterial artificial chromosomes) will be used to perform array CGH (Comparative genomic hybridization) of prostate tumor samples. Successful identification of a gene on the Y chromosome will provide a marker that may aid in the diagnosis and prediction of prognosis of prostate cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2003
- Accession Number
- ADA421200
Entities
People
- Susan L. Naylor
Organizations
- University of Texas at San Antonio