TGF(Beta)1 Regulation of Matrix Metalloproteinase-9 in Human Prostate Cancer Metastasis
Abstract
The purpose of this project is to identify and test portions of the 3 prime untranslated region of the Matrix Metalloproteinase-9 mRNA responsible for TGFBETA-induced increases in mRNA stability. This is to be accomplished by generating constructs of the 3'UTR and comparing them with the entire MMP-9 coding sequence in transfection experiments. This past year I obtained a modified SOW, in which part of our second task was changed to allow us to use our MMP-9 3'UTR deletion constructs in vitro invasion assays and then measure MMP-9 expression and inhibition of invasion as end points. Western blot analyses and zymography using one cell line suggest a portion of the 3 UTR, when overexpressed, can reduce TGFBETA-simulation of MMP-9. Northern blots were being conducted. A serious problem has arisen with this research project. I have recently obtained a faculty position elsewhere. Upon learning of my departure, NDSU has given me only restricted access to my office and laboratory. Notebook and all reagents pertinent to this project have been seized. As I write this report, I am not able to communicate with the student who worked on this project, nor have I been allowed access to her data, notebook or reagents.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2003
- Accession Number
- ADA421319
Entities
People
- Inder Sehgal
Organizations
- North Dakota State University