Development of a Novel Ligand Binding Assay for Estrogen Receptor

Abstract

Nuclear receptors undergo conformational changes when they bind ligands. It should be possible to monitor these changes in vivo using energy transfer between fluorophores. The existence of inherently fluorescent proteins such as the variants of jellyfish green fluorescent protein (GFP) suggests that this problem may be approached by making fusions of these proteins to nuclear receptors. We set out to study this problem using the estrogen receptor (ER), a nuclear receptor known to undergo a conformational change upon ligand binding.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 2002
Accession Number
ADA421346

Entities

People

  • Arthur C. Chung

Organizations

  • Baylor College of Medicine

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Abstracts
  • Androgen Receptors
  • Biomedical Research
  • Biomolecules
  • Breast Cancer
  • Cell Line
  • Cells
  • Chemical Compounds
  • Culture Techniques
  • Energy
  • Energy Transfer
  • Estrogens
  • Gene Expression
  • Hormones
  • Hormones Hormone Substitutes And Hormone Antagonists
  • Mammary Glands
  • Proteins

Fields of Study

  • Chemistry

Readers

  • Breast cancer cell signaling and growth regulation.
  • Molecular Genetics
  • Oncology and Biomarker-Based Cancer Detection.