Cloning and Characterization of Genes that Inhibit TRAIL-Induced Apoptosis of Breast Cancer Cells

Abstract

TRAIL is a tumor necrosis factor family member that can specifically induce apoptosis of cancer cells but not of normal cells (1-5). However, some cancer cells are resistant to TRAIL-induced apoptosis (3, 4, 6-13). The purpose of this proposed study is to clone and characterize such inhibitory genes of TRAIL-induced apoptosis. To this end, following specific aims are proposed: #1. To clone genes that inhibit TRAIL- induced apoptosis of MCF7 cells by a subtractive hybridization screening approach and an expression/functional cloning approach using retroviral cDNA libraries; #2. To functionally characterize genes identified from specific aim #1.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 2003
Accession Number
ADA421784

Entities

People

  • Hong-bing Shu

Organizations

  • National Jewish Health

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Apoptosis
  • Biomedical Research
  • Breast Cancer
  • Cell Line
  • Cells
  • Classification
  • Diseases And Disorders
  • Electronic Mail
  • Genetic Structures
  • Hybridization
  • Inhibitors
  • Necrosis
  • Neoplasms
  • Proteins
  • Resistance

Fields of Study

  • Biology

Readers

  • Cellular and Molecular Pathways of Apoptosis.
  • Molecular Biology and Genetics