Discovery of Novel Metastasis Genes in Breast Cancer
Abstract
Under this proposal we plan to identify genes that drive the homing of invasive primary breast cancer to its metastatic sites. For the discovery of such genes, in vivo phage display of breast cancer cDNA libraries will be used. Validation will be achieved by expression monitoring in histological sections as well as by functional assays. In a first series of experiments we established the methodology for in situ hybridization of tissue microarrays containing paraffin-embedded breast cancers for homing genes that were discovered using the liver as a target organ. No distinct expression was found for breast cancer vs. non-cancerous breast tissues. It is planned to next select homing genes with the breast cancer cell library derived from MDA-MB 231 cells. Then, homing genes derived from that library will be screened against the breast cancer tissue microarray.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2003
- Accession Number
- ADA421786
Entities
People
- Anton Wellstein
Organizations
- Georgetown University