Tropomyosin-1 A Novel Class II Tumor-Suppressor and a Biomarker of Human Breast Cancer
Abstract
Previous research from this laboratory indicated that 1. the expression of Tropomyosin-1 (TMI), a microfilament- associated protein, is abolished from many human breast carcinoma cells, and; 2. that TMI is suppressor of the malignant transformation. These data led to the hypothesis that TMI plays an important role in mammary carcinogenesis. Therefore, we investigated whether TMI could serve as a biomarker of breast cancer, and TMI could function as a suppressor of the malignant growth of breast cancer cells. Our results show that TMI is downregulated in breast tumors (Objective 1). We demonstrated that TMI is a suppressor of the malignant growth phenotype of MDA MB 231 cells, indicating that TMI is a general suppressor of the transformed growth (Objective 2). To assess the structure-function relationship of tumor suppression by TMI, we constructed chimeric and variant TMI proteins. By employing a variant TMI that contains an aminoterminal extension, we show that the aminoterminal integrity is important for TMI-mediated tumor suppression (Objective 4).
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2003
- Accession Number
- ADA421793
Entities
People
- Gaddamanugu L. Prasad
Organizations
- Wake Forest University