Enzymatic Detoxification of Chemical Warfare Agents

Abstract

We succeeded in completing our objectives for this research project. These accomplishments include the following: (a) synthesis and development of a flexible organophosphate substrate analog library for each of the stereoisomers of GB, GD, and VX. These compound have been utilized in the high throughput screening of mutant protein libraries; (b) site-directed mutagenesis of each amino acid within the active site of the bacterial phosphotriesterase; (c) development of mutagenic protocols for the efficient construction of protein libraries with altered catalytic properties; (d) development of high throughput spectrophotometric screens for characterization of individual mutant proteins derived from the enzyme libraries.

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Document Details

Document Type
Technical Report
Publication Date
Dec 31, 2003
Accession Number
ADA421843

Entities

People

  • Frank M Raushel

Organizations

  • Texas A&M University

Tags

Communities of Interest

  • Human Systems

DTIC Thesaurus Topics

  • Amino Acids
  • Biochemistry
  • Chemical Compounds
  • Chemical Warfare
  • Chemical Warfare Agents
  • Chemistry
  • Construction
  • Detoxification
  • Mutant Proteins
  • Nerve Agents
  • Organophosphates
  • Proteins
  • Stereoisomers
  • Substrates
  • Throughput
  • Warfare

Readers

  • Analytical Chemistry
  • Molecular Genetics
  • Molecular and Cellular Biochemistry