Rapid Detection of Cellular Responses to Biological Agents
Abstract
Our program objective is to develop simple and rapid methods for detecting, at a cellular level, individual responses to environmental stresses elaborated by exposure to infectious agents such as bacteria and viruses. Our methods are based on transcript profiling and post-translational modification of proteins involved in signal transduction. Our hypothesis is that human cells respond to infectious insults to a genetically predetermined extent by stimulating the expression of sets of genes and activating signaling pathways that provide a specific signature for a given agent. We propose that this response will determine the outcome of the infection. We will test this hypothesis by developing custom cDNA and protein arrays designed to detect cellular responses to infectious agents. These will be tested using RNA and protein isolated from tissues sources most likely to be exposed. Our methods will allow development of rapid quantitative detection devices to measure exposure and response to biological warfare, bioterrorism or emerging agents enabling appropriate triaging and medical intervention to save lives and to avoid unnecessary treatments.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2004
- Accession Number
- ADA421869
Entities
People
- Bryan R. Williams