Aberrant Gene Expression in NF1-Mediated Oncogenisis

Abstract

Recent genetic understanding has identified the gene NF1 to be disrupted or mutated in patients affected with NF1 resulting in reduced expression of the protein neurofibromin. One function of NF1 has shown that it acts to negatively regulate, or turn off, positive signals in the cell that direct the cell to proliferate. Specifically, NF1 turn the positive signals relayed by a protein called Ras. However, other studies have shown that NF1 may negatively regulate cell proliferation by mechanisms other than through inactivation of Ras. These other mechanisms however, are currently unknown. The purpose of the proposed study was to determine what changes in gene expression occur in cells from a patient with neurofibromatosis when we force these cells to express neurofibromin. The study was designed to identify and determine genes deregulated by NF1 in a Ras-dependent and Ras-independent manner. Taken together, these studies were designed to contribute to our understanding of the function and downstream effects NF1 has on the cell. In addition, determining the downstream nuclear events that occur in response to NF1 may provide novel targets for the treatment of NF1 associated cancers.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2003
Accession Number
ADA421873

Entities

People

  • Janiel M. Shields

Organizations

  • University of North Carolina at Chapel Hill

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Antibodies
  • Assays
  • Bioassay
  • Biomedical Research
  • Cancer
  • Cell Line
  • Cells
  • Chemistry
  • Diseases And Disorders
  • Gene Expression
  • Genes
  • Genetic Phenomena
  • Neoplasms
  • Neurofibromatosis
  • Neuromuscular Diseases
  • Proteins

Fields of Study

  • Biology

Readers

  • Molecular and Cellular Biology

Technology Areas

  • Biotechnology