Blood-Brain Barrier Transport of Uranium
Abstract
Studies in Year 2 continued according to the original plan. Studies were carried out in RBE4 cells and in primary astrocyte cultures. In both cell types, there was a significant increase in 54manganese uptake due to the addition of uranium to the media. Incubation with uranium did not significantly alter DMT-1 may not be the putative transporter for uranium across the blood-brain barrier. In addition studies, which were delayed by the lack of availability of DU pellets, we implanted rats in the gastrocnemius muscle with 10 pellets in each leg for a total of 20 pellets. The following doses were administered: sham=20 tantalum (Ta) pellets; low=16 Ta and 4 du; medium=10 Ta and 10 du; high=4 Ta and 16 du. The three-month cohort will be analyzed shortly for uranium brain levels, as well as DMT-1 expression in various brain regions. Rats have been monitored (e.g.; body weights, coat quality), and there are no noted differences in body weights in either the three-month or six-month cohorts (Figure 4) or physical appearance have occurred in rats receiving surgery (both Ta and du pellets) compared to non-surgical controls.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2003
- Accession Number
- ADA422003
Entities
People
- Michael Aschner
Organizations
- Wake Forest University