Roles of Steroid Receptor Coactivators in Breast Cancer
Abstract
The purpose of this study is to determine the roles of steroid receptor coactivators in breast cancer. The scope of the research is assessing the expression of SRC-1 family members (SRC-1, TIF2, and AIB1) during breast cancer progression using breast cancer tissue and adjacent normal tissue obtained from Indiana University Tissue Procurement Facility. In addition, functions of these coactivators have been studies during this report period. We have constructed adenovirus expressing these three coactivators. We found that AIB1 protein was overexpressed in human breast cancer specimens, as compared to adjacent normal breast tissue. In addition, increased expression of AIB1 in human breast cancer cells stimulated the S phase entry during cell cycle progression, indicating the involvement of AIB1 in cell cycle control and cell growth of human breast cancer cells. Furthermore, overexpression of AIB1 dramatically increased the phosphorylation of Akt and Akt kinase activity in human breast cancer cells. These studies provide important insights how AIB1 works in human breast cancer cells to promote breast cancer progression.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2003
- Accession Number
- ADA423247
Entities
People
- Meei-huey Jeng
Organizations
- Indiana University