FGF Activation and Signaling in Breast Cancer
Abstract
Fibroblast growth factor binding protein (FGF-BP1) is a crucial molecule that acts to chaperone active FGFs to receptors, thus propagating angiogenic signals for the development of new vasculature. We have shown that FGF-BP1 is expressed in head and neck, skin, cervical, and lung squamous cell carcinomas. A second family member, FGF-BP2 has been identified in our lab and is present in mammary tissue. In this grant, we hypothesized that FGF-BP2 acts in a similar pro-angiogenic capacity as FGF-BP1. The aims were 1) to produce recombinant FGF-BP2 and test its effect on signal transduction, and 2) to study the expression of FGF-BP2 during mouse mammary gland development and carcinogenesis. To date, we have isolated human FGF-BP2 cDNA and protein and confirmed its ability to modulate FGF2. However, we have yet to discover the murine homologue to FGF-BP2. We have identified a third family member, FGF-BP3 in human and mouse. Accordingly, we have adjusted our focus to the characterization of FGF-BP3 activity and expression while continuing our search for murine FGF-BP2. FGF-BP3 has been shown to complex with FGF2, promote increased proliferation, MAPK activation, and anchorage-independent growth in SW-13 adrenal carcinoma cells, and is present in high levels in mouse embryonic tissue.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2003
- Accession Number
- ADA423280
Entities
People
- Matthew R. Swift
Organizations
- Georgetown University