Characterization of a Dopaminergic Stimulatory Factor Derived From Monoclonal Cell Lines of Striatal Origin
Abstract
Unique dopaminergic modulatory polypeptide factors have been obtained form the lysate of an immortalized monoclonal cell derived for the mouse striatum (X61). Partially purified preparations of the lysate are capable of increasing the dopamine content of hybrid monoclonal dopaminergic cells of mesencephalic origin and preventing the loss of primary dopaminergic neurons in the absence of target cells. The monoclonal derived dopaminergic stimulatory factor has undergone a 50,000-fold purification sufficient to permit mass spectrographic and amino acid sequence analysis of the purified fractions. Based on this data, polypeptide fractions of known amino acid composition have been synthesized and are being examined for stimulatory activity in monoclonal dopaminergic cells and for the ability to prevent loss of primary dopaminergic neurons in the absence of striatal target cells. The availability of polypeptides of know molecular structure will also permit assessment of whether such agents are capable of preventing of reversing the motor deficits seen in experimental models involving loss of the dopaminergic neurons of the nigrostriatal projection. Polypeptide factors of low molecular weight which modulate neuronal monoaminergic function are of considerable interest in terms of their therapeutic potential in a variety of neurologic disorders including Parkinson's disease.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2003
- Accession Number
- ADA423626
Entities
People
- Alfred Heller
Organizations
- University of Chicago