Molecular Engineering of Vector-Based Oncolytic and Imaging Approaches for Advanced Prostate Cancer
Abstract
Hormone refractory and metastatic prostate cancer are not well understood. Better animal models, diagnostic and treatment modalities are sorely needed for these advanced stages of disease. We have coupled non-invasive optical imaging to develop metastatic prostate cancer animal models, and vector-based diagnostic and therapeutic approaches. A highly potent and prostate-specific transcriptional regulatory system (TSTA) has been utilize dot restrict the expression of our adenoviral vector specifically to prostate or prostate cancer cells. In the diagnostic approach, this TSTA system will be applied to express optical imaging reporter gene. Alternatively, the TSTA system will be applied to regulate the expression of viral replication proteins in the therapeutic approach. In doing so, the viral amplification and cell lysis will be directed in a prostate-specific manner. The progress of this project has been interesting and according to plan in the first year. Several human prostate xenograft models have been marked with the optical luciferase reporter gene. This approach allows facile monitoring of cancer dissemination. We observed distinctily different metastatic potentials in different models. These metastatic animal models will be the bases for us to investigate the efficacy of our diagnostic and oncolytic therapeutic viral vectors.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2004
- Accession Number
- ADA424027
Entities
People
- Lily Wu
Organizations
- University of California, Los Angeles