An MR Contrast Agent for Intra-Prostatic Imaging of Prostatic Cancer
Abstract
The goal of the current research is the development of a magnetic nanoparticle based MR contrast agent that binds the gastrin releasing peptide receptor, a molecular marker associated with neoplasia. In the first year of this proposed work a series of bombesin derivatives have been synthesized and their binding to the GRP receptor quantitated by displacement of radioiodinated bombesin. Positively charged derivatives have lower LC50s, suggesting that the environment around the binding pocket is negatively charged and hydrophilic. Selected peptides have been conjugated to magnetic nanoparticles with various numbers of peptides per nanoparticle. A non-isotopic, enzyme immunoassay method, based on the ability to quantify fluorescein, has been developed to replace the assay using receptor binding and displacement of radioiodinated peptides.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2004
- Accession Number
- ADA424091
Entities
People
- Lee Josephson
Organizations
- Massachusetts General Hospital