INT6 is a Potential Tumor Suppressor for Breast Cancer
Abstract
This project tests the hypothesis that Int6 regulates the 26s proteasome and acts as a tumor suppressor for breast cancer. With the support of this grant, we were able to complete a number of studies in a genetic model system (yeast), thus firmly establishing the fact that Int6 regulates the proteasome. To further our studies in mammalian cells, the key experiment proposed in this project period is to create cell lines in which Int6 can be inactivated. The most direct and powerful way of doing this is to use RNAi to knock-down 11VT6 expression. We have started testing several oligos but so far have not yet successfully knocked down INT6 expression. To properly detect INT6 expression, we have obtained two antibodies against Int6 from colleagues. In the mean time, we have made anti peptide antibody against Int6 and are further improving its specificity for immunoblots. As an alternative approach to inactivate Int6 activities, we are constructing cell lines that express C-terminally truncated Int6, which may be dominant negative. We are performing RT-PCR now to screen a number of clones that may stably express these proteins.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2004
- Accession Number
- ADA424092
Entities
People
- Eric C. Chang
Organizations
- Baylor College of Medicine