Real-Time PCR Analysis of Peripheral Blood: A Non-Invasive Technology That Can Be Used to Assess Tumor Burden in Breast Cancer Patients

Abstract

The molecular detection of circulating tumor cells (CTC) in breast cancer patients has been limited by two factors: 1) low concentration of CTC in peripheral blood, and 2) normal lymphocytes express some cancer-associated genes at detectable levels. In this study, we investigated the ability of a novel buoyant density gradient centrifugation (BDGC) medium (Oncoouick, HEXAL Gentech) to enrich CTC prior to real-time RT-PCR analysis.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2003
Accession Number
ADA424096

Entities

People

  • Michael Mitas

Organizations

  • Medical University of South Carolina

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Blood
  • Blood Cells
  • Breast Cancer
  • Cell Line
  • Cells
  • Detection
  • Diagnostic Imaging
  • Diseases And Disorders
  • Gene Expression
  • Lymphocytes
  • Neoplasms
  • Pcr Testing
  • Pilot Studies
  • South Carolina
  • Standards
  • Virotherapy

Fields of Study

  • Biology

Readers

  • Oncology and Biomarker-Based Cancer Detection.