Development of COMET-FISH Method for Rapid Identification of Persons at Risk for Breast Cancer Development. Addendum

Abstract

A proportion of breast cancers have genetic alterations at 17q11.2-q12 (HER-2/neu), 20q13.2 (ZNF217 gene) and 17p13.1 (p53). We have documented global genetic instability within malignant breast cancer cell lines (MCF-7; MDA-MB-468 and CRL-2336) by coupling the DNA-damage Comet assay to fluorescence in situ hybridization (Comet-FISH). Gene-specific instability at p53 and HER-2 loci was also observed. Following genotoxic insult (IR and H2O2), we found that p53 locus was capable of rapid and preferential repair when compared to the HER-2 locus. Comet-FISH is a rapid and easy assay which is amenable for the study of gene- specific instability or DNA repair in tissues relating to cancer risk or progression.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2004
Accession Number
ADA424123

Entities

People

  • Robert Bristow

Organizations

  • University Health Network

Tags

DTIC Thesaurus Topics

  • Breast Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chromosome Aberrations
  • Fluorescence
  • Genetics
  • Genomic Instability
  • Growth Factors
  • Health Services
  • Hybridization
  • Instability
  • Ionizing Radiation
  • Medical Personnel
  • Neoplasms
  • Oncology
  • Tumor Cell Line

Fields of Study

  • Biology

Readers

  • Molecular and genetic basis of cancer.
  • Oncology (Cancer Research).
  • Oncology and Biomarker-Based Cancer Detection.

Technology Areas

  • Biotechnology