Determination of a Unique Pattern of Gene Expression in Node Positive Breast Cancer Using Serial Analysis of Gene Expression (SAGE)

Abstract

The main hypothesis of this project is that a specific genetic expression pattern for lymphogeneous spread exists in a small fraction of cells in primary breast cancer. The Principal Investigator (PI) believes that this expression pattern will be the same in primary tumors from different women. These patterns could then be exploited to determine the lymph mode status of a primary breast cancer. This DOD concept award extension investigated whether a genetic signature in the primary tumor could be developed for lymph node positive breast cancer. Using a combination of serial analysis of gene expression (SAGE) and microarray techniques, a preliminary genetic signature has been developed. Further experiments are in progress to refine the genetic signature and confirm the overexpression of certain genes that predict for lymph node positive breast cancer.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2003
Accession Number
ADA424196

Entities

People

  • Adam M. Brufsky

Organizations

  • University of Pittsburgh

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Breast Cancer
  • Cancer
  • Carcinoma
  • Dna Microarrays
  • Gene Expression
  • Genes
  • Genetic Phenomena
  • Lymph Nodes
  • Lymphatic System
  • Microarray Analysis
  • Neoplasms

Readers

  • Applied Combinatorial Optimization and Logic Circuit Design.
  • Medical Imaging.
  • Prostate Cancer Biology.

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech