Regulation of Genes Controlling Carbohydrate Metabolism in the Heart of a Hibernating Mammal
Abstract
The project funded by this ARO grant is to characterize genes that are responsible for the physiological changes that occur during hibernation in the heart of the thirteen-lined ground squirrel (Spermophilus tridecemlineatus). We focused on the heart because it is a contractile organ that must continue to work despite physiological conditions that would be lethal to most mammals. We used a PCR-based gene expression screen to isolate cDNAs of genes showing increased levels of expression in the heart during hibernation. In the process we learned a considerable amount about the function of various gene products during hibernation. Specifically, we learned about the genes responsible for the switch from a carbohydrate-based metabolism to a fat-based metabolism and their regulation We examined novel genomic structures, analyzed the evolution of genes required for hibernation and showed high homology to human orthologs. Finally we demonstrated how fat in the form of triacylglycerols can be hydrolyzed at temperatures as low as O C.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 18, 2004
- Accession Number
- ADA424253
Entities
People
- Matthew T Andrews
Organizations
- University of Minnesota Duluth