Peptide-Mediated Transduction of Proteins and Nucleic Acids to Prevent and Treat Experimental Prostate Cancer
Abstract
Our goal in this project is to prevent the occurrence of bone metastasis in early experimental prostate cancer using protein transduction: the ability of small peptides to facilitate the entry of large biologically active fusion protein cargos into cells. The hypothesis to be tested is that protein transduction can deliver therapeutic proteins to skeletal tissues and bone marrow in such a manner that they may facilitate the apoptotic, or programmed cell death of cancerous cells and tissues of the bone. Prostate cancer is lethal and incurable once it has metastasized to the bone. We and others have previously shown that protein transduction can allow many proteins into organs previously unaccessible to other delivery methods (drugs, viral vectors etc). We have constructed unique protein-transduction domains (PTD5 and Lys8) which we have demonstrated efficiently facilitate the entry and activity of the pro-apoptotic peptide Smac34 into prostate cancer cell lines. Protein transduction may prove to be a useful drug delivery method of biologically active proteins in cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2003
- Accession Number
- ADA424262
Entities
People
- Janey D. Whalen
Organizations
- University of Pittsburgh