Image Guidance and Assessment of Radiation Induced Gene Therapy

Abstract

Image guidance and assessment techniques are being developed for combined radiation/gene therapy, which utilizes a radiation-inducible gene promoter to cause expression of tumor necrosis factor alpha in irradiated tissues. TNF attacks vasculature, increasing the tumor killing effect of radiation. The radiation confines TNF toxicity to the irradiated region. This therapy has proven effective in several animal tumor models and phase I clinical trials. This project is developing imaging to visualize the effects of the combined modality therapy, by combining electron paramagnetic resonance imaging (EPRI), mapping oxygen concentration in 3D in vivo, and nuclear magnetic resonance imaging (MRI), measuring quantities such as vascular permeability and perfusion rate that reflect the status of vasculature. EPRI and MRI of prostate tumors in mice and rats will be conducted. Fusing the two sets of images from the same tumor before, during and after therapy, an image-derived signature will be developed which identifies regions responding well to therapy. In the final stage, this information will be used as the basis of an adaptive treatment regimen where regions that have responded less well will be given a boost, via image guided injection of additional gene vector prior to the next fraction of radiation.

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Document Details

Document Type
Technical Report
Publication Date
Feb 01, 2004
Accession Number
ADA424653

Entities

People

  • Charles A. Pelizzari

Organizations

  • University of Chicago

Tags

DTIC Thesaurus Topics

  • Anatomy
  • Blood
  • Blood Vessels
  • Cell Physiological Processes
  • Electron Paramagnetic Resonance
  • Gene Therapy
  • Health Services
  • Magnetic Resonance
  • Magnetic Resonance Imaging
  • North America
  • Paramagnetic Resonance
  • Prostate
  • Prostate Cancer
  • Radiation
  • Resonance
  • Resonators
  • Tissues

Fields of Study

  • Medicine
  • Physics

Readers

  • Medical Imaging.
  • Nuclear and Radiation Engineering.
  • Oncology (Cancer Research).

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech
  • Microelectronics