Mitochondrial Structure and Reactive Oxygen Species in Mammary Oncogenesis
Abstract
Oxidative stress may play a role in human oncogenesis, including breast cancer. The mitochondria are most frequent sources of reactive oxygen species (ROS) responsible for most oxidative stress. This project evaluates the role of mitochondrial abnormalities in oxidative stress in breast cancer development. An advanced cre-LoxP gene activation strategy will be used to over-express mutant mitochondrial complex II subunits in the mammary glands of transgenic mice. These mutations are responsible for elevated levels of ROS in the cells. The transgenic mice will be characterized in terms of mitochondrial functions, ROS productions and oncogenesis in the mammary glands. The effects of oxidative stress in other transgenic mouse models of breast cancer will be evaluated. Bi- or tri-transgenic mice will be generated by crossing and analyzed in terms of their breast cancer development, in the presence or absence of mitochondrial mutant gene and hence oxidative stress. This study should provide significant information regarding the role of oxidative stress in breast cancer development and progression, and insights on whether antioxidants are beneficial in prevention and treatment of such important cancer in women.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2004
- Accession Number
- ADA425067
Entities
People
- Yun-fai C. Lau
Organizations
- Northern California Institute for Research and Education