Enhancing the Immunogenicity of a Dengue-2 DNA Vaccine with Adjuvants and Anti-FCgammaRI Antibodies
Abstract
The objective is to use adjuvants to enhance the immunogenicity of a dengue (DEN) DNA vaccine. The adjuvants to be evaluated in this project include aluminum phosphate, tetanus toxoid and anti-FcyR monoclonal antibodies chemically linked to the DNA vaccine. Initial attempts to link the anti-FcyR monoclonal antibody to the DNA vaccine resulted in a poor efficiency in the linkage reaction. An alternative method of adding the thiol group (EndTag Nucleic Acid Labeling System) to the linearized DNA should increase the reaction efficiency. Experiments were initiated to evaluate the ability of tetanus toxoid (TT) to enhance the immunogenicity of the DEN DNA vaccine. The antibody responses to the vaccine given IM were enhanced slightly by combining it with TT. However, the ELISA and neutralizing antibody levels were inferior to the levels obtained with ID administration of the vaccine alone. Aluminum Phosphate was demonstrated to slightly enhance anti-dengue neutralizing antibody responses, only when the DNA vaccine was given ID at sub optimal doses. There was essentially no effect when the vaccine was administered IM.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2003
- Accession Number
- ADA425592
Entities
People
- Kevin R. Porter
Organizations
- Naval Medical Research Center