Do Perturbed Epithelial-Mesenchymal Interactions Drive Early Stages of Carcinogenesis
Abstract
This research project had three specific tasks. The first was to determine which tissue is the target of the chemical carcinogen N-nitrosomethylurea in the rat mammary gland. This task was scheduled to occupy the first 18 months of support. The second task included screening and counting lesions and performing morphometric data analysis of whole mounts (branching pattern, relative abundance of the different ductal and alveolar structures). These studies aimed to identify the changes occurring between the time of exposure and the appearance of neoplasias. We also planned to analyze BrdU incorporation, and identify changes in components of the extracellular matrix. Finally, during years two and three we were supposed to explore the specific roles of hyaluronan and emmprin, two molecules that are enriched in tumors and involved in tumor-stromal cell interactions as mediators of neoplastic initiation and progression.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2004
- Accession Number
- ADA425598
Entities
People
- Carlos Sonnenschein
Organizations
- Tufts University