Mechanism of FADD-DN-Induced Apoptosis in Normal Breast Cells

Abstract

Normal cells undergo apoptosis in response to inappropriate growth signals or the lack of oven survival signals. Tumor cells possess defects in apoptosis regulatory pathways and do not undergo apoptosis in these situations. Because FADD is an essential component of receptor mediated apoptosis, a dominant-negative version (FADD-DN) is able to block apoptosis induced by death ligands in many cell lines. While studying FADD signaling, our laboratory made the surprising discovery that FADD-DN can induce apoptosis in normal breast epithelial cells. Because FAD%DN induces apoptosis in normal but not cancerous breast epithelial cells, we hypothesize that FADD-DN interacts with one or more proteins expressed in breast epithelia. Since breast tumor cells do not die in response to FADD% DN, the potential FADD-DN interacting partners are likely to be involved in carcinogenesis. Since defects in apoptotic pathways are a prerequisite to cancer, understanding the nature of these defects may bring about potential treatments. FADD-DN signaling presents a novel apoptotic pathway that is fundamental in normal breast epithelia, but not breast cancer cells. Components of this pathway may identity potential therapeutic targets that allow the reactivation of this apoptotic response in cancer cells

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 2004
Accession Number
ADA425715

Entities

People

  • Lance R. Thomas

Organizations

  • Wake Forest University

Tags

DTIC Thesaurus Topics

  • Apoptosis
  • Biomedical Research
  • Breast Cancer
  • Buildings And Structures
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Epithelial Cells
  • Epithelium
  • Hybrid Systems
  • Mutations
  • Neoplasms
  • North Carolina
  • Programmed Cell Death
  • Security

Fields of Study

  • Biology

Readers

  • Military Logistics and Supply Chain Management
  • Molecular Biology and Genetics