Microlocalization and Quantitation of Risk Associated Elements in Gleason Graded Prostate Tissue
Abstract
Epidemiological and laboratory studies show that boron, selenium and zinc reduce prostate cancer risk whereas calcium and cadmium increase risk. The objective of this proposal is to determine the concentration and location of these elements in normal and tumor tissue. Specific aims include: (1) preparation of four grades of prostate tissue, (2) determination of tissue concentrations of: B, Ca, Cd, Se, and Zn; and (3) determination of tissue and cellular distribution of these elements using NanoSIMS ion microscope at Lawrence Livermore National Laboratory (LLNL). Progress toward specific aim 1 includes preparing matched samples of normal tissue with graded tumor tissue from pathological tissue at prostatectomy. Progress toward specific aim 2 includes method development and analysis of 23 paired samples of normal and Gleason graded tumor tissue. Results were submitted in October for presentation at the April 2004 Experimental Biology Meetings in Washington, D.C. The data do not support an association between Gleason scores and the elements concentration in prostate tissue, but show considerable variability in the population. Work toward specific aim 3 has concentrated on methods development for NanoSIMS ion microscopy. Two students will undergo training at LLNL to become expert in the use of the NanoSIMS ion microscopy.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2004
- Accession Number
- ADA425860
Entities
People
- Curtis Eckhert
Organizations
- University of California, Los Angeles