Identification of Novel Genes Affected by Gamma Irradiation Using a Gene-Trapped Library of Human Mammary Epithelial Cells
Abstract
We propose that the expression of several unknown genes is affected by gamma radiation. Abnormal expression of these genes may be one of the steps in breast carcinogenesis induced by radiation. We plan to establish an assay that will allow us to screen for breast cells that contains a single mutation by gene trapping. We will be able to detect changes in the expression of a gene upon treatment with different doses of radiation. These radiation-responsive genes will be identified through the rapid amplification of cDNA ends procedure and sequenced. Cells that are affected by radiation will be isolated and analyzed to see if the changes can lead to transformation of the normal breast epithelial cell into a neoplastic cell. This assay may prove to be a powerful tool in the identification of novel genes that are affected by gamma irradiation in the early stages of breast cancer progression. The 3'RACE protocol has recently been completed and thirty one genes potential genes were sequenced. Of these, six candidate genes were found. The include: human creatine kinase gene, human androgen receptor, human DORA reverse strand protein 1 (DREVl), human eukaryotic translation elongation factor 1 beta 2 (EEFlB2), human ribosomal protein L27, and human DNA clone RPll-290F20 on chromosome 20. These genes will be further analyzed for their transformation properties of human mammary epithelial cells as discussed in the
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2004
- Accession Number
- ADA425893
Entities
People
- Jennifer L. Malone
- Robert Ullrich
Organizations
- Colorado State University