Markers of Increased Risk in Pre-Invasive Breast Cancer
Abstract
The understanding of the risk of recurrence and progression of breast cancer, particularly, from a pre-invasive lesion is evasive and challenging. Our goal has been to identify changes in early breast cancer using techniques to profile global gene expression. In previous work, we have employed cDNA array techniques to identify relatively small changes in gene expression between low and high risk pre-invasive cancer. We have therefore optimized the alternative SAGE (serial analysis of gene expression) technique to also apply to this problem and compare with our cDNA results. We have tested SAGE initially in a small pilot experiment, using transfected cell lines that differ in their expression of the psoriasin gene, that is commonly overexpressed in pre-invasive cancer. A pilot experiment using two SAGE libraries showed that differences can be detected and larger experiment is now underway. Clones with inserts have been screened by PCR and agarose gel analysis and are now being sequenced. We anticipate that this experiment will establish the capability of this assay to detect differential gene expression and lay the foundation for its application to pre-invasive tumor samples, and may identify gene that are overexpressed in pre-invasive disease.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2004
- Accession Number
- ADA425897
Entities
People
- Peter H. Watson
- Soma Mandal
Organizations
- University of Manitoba