A Genomic Approach to Identifying Novel Targets for Early Detection and Intervention of Prostate Cancer
Abstract
Early detection and intervention is key to a favorable prognosis in prostate cancer. Despite advances in the detection and treatment of prostate cancer, the mortality rate remains high. To improve survival, early detection and treatment strategies tailored to pre-invasive prostate cancer are required. We propose to catalog genetic alterations associated with the developmental stages of disease for use as diagnostic tools and to identify the critical genes that drive the transformation of premalignant lesions to tumors for use as molecular targets for novel treatment design. The combination of laser capture microdissection (efficient isolation of specific cell types form hundreds of specimens) and SMAL DNA fingerprinting technology (high-through put analysis of genomic targets using minute quantities of DNA yielded form the microdissected cells) will facilitate systematic comparison of samples in various stages of disease development. By the end of this work, we will have identified a set of genetic loci (and genes) by virtues of their frequency of alteration in premalignant lesions and subsequent in low-grade tumors. We will have established a publicly accessible "genetic alterations in prostate cancer database which catalogs somatic changes present in the various stages of cancer progression. Such information contribute to the fundamental understanding of prostate cancer pathogenesis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2003
- Accession Number
- ADA425926
Entities
People
- Wan L. Lam
Organizations
- BC Cancer Agency