Cloning and Characterization of Genes That Inhibit TRAIL-Induced Apoptosis of Breast Cancer Cells

Abstract

TRAIL is a tumor necrosis factor family member that can specifically induce apoptosis of cancer cells but not of normal cells. However, some cancer cells are resistant to TRAIL-induced apoptosis. The purpose of this proposed study is to clone and characterize such inhibitory genes of TRAIL-induced apoptosis. Using cDNA subtraction and retroviral cDNA-based expression cloning approaches, we have obtained more than multiple candidate clones of TRAIL-inhibitory genes. Among the candidate clones, the short splice form of Casper/c-FLIP (Casper-S were shown to confer resistance to TRAIL-induced apoptosis. Casper deficient embryonic cells were sensitive to TRAIL-induced apoptosis. Re- introduction of Casper-S into Casper deficient cells conferred resistance to TRAIL-induced apoptosis. This project has identified and validated Casper-S as a major cellular inhibitor of TRAIL-induced apoptosis.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 2004
Accession Number
ADA425962

Entities

People

  • Hong-bing Shu

Organizations

  • National Jewish Health

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Apoptosis
  • Biological Sciences
  • Breast Cancer
  • Calcium Compounds
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Genes
  • Genetic Structures
  • Genetics
  • Inhibitors
  • Necrosis
  • Neoplasms
  • Proteins
  • Resistance

Fields of Study

  • Biology

Readers

  • Cellular and Molecular Pathways of Apoptosis.
  • Molecular Biology and Genetics