Development of a Novel Tissue Slice Culture Model of Human Prostate Cancer
Abstract
In year 2, we have improved methods of reproducibly obtaining prostate tissue cores for tissue slice culture, of trimming the block face of fixed and embedded tissue slice cultures so as not to lose tissue for analysis, and have tested a number of fixation methods for optimum preservation of antigenicity. We have added additional antibodies to our repertoire to evaluate maintainence of structure and function of tissue slice cultures. Clinically relevant drugs with known activity in monolayer cell cultures and in patients have been tested on tissue slice cultures, and preliminary results suggest similar activity in tissue slices. This data supports our premise that tissue slice cultures can provide a realistic preclinical model to test experimental drugs. Currently, we are exploring immunoblot analyses of protein lysates from tissue slice cultures as a more quantitative and faster method of evaluating effects of drugs and other compounds on tissue slice cultures. Promising candidates can then be further analyzed by the more tedious technique of immunohistochemistry of fixed sections.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2004
- Accession Number
- ADA425981
Entities
People
- Donna M. Peehl
Organizations
- Stanford University