Mutagen Sensitivity Apoptosis and Polymorphism in DNA Repairs as Measures of Prostate Cancer Risk
Abstract
This proposal evaluates interindividual differences in the response to genotoxic stress as prostate cancer risk factors. To this end we use measurements of mutagen sensitivity, apoptosis, comet assay, and single nucleotide polymorphisms in DNA repair genes OGG1 and XRCCl. These biomarkers are evaluated in 100 prostate cancer cases and 100 controls matched on age and race in order to measure response to bleomycin exposure is short-term cultured lymphocytes to define prostate cancer risk. During the second year of funding, a study coordinator recruited 21 prostate cancer cases and 20 matched controls at the Georgetown University Hospital. A research assistant created a sample repository consisting of serum, plasma, buffy coat, urine, toenail clipping and saliva for every participant. We also created a computerized database of the samples in Microsoft Access. The research assistant measured mutagen sensitivity in all the subjects and determined the mean breaks in lymphocytes exposed to bleomycin in cases (mean 0.88 SD 0.32) and controls (mean 0.74 SD 0.34). We continue to optimize the apoptosis and comet assay protocols to measure DNA repair kinetic and cell death in exposed cells. We expect to proceed rapidly with the case-control study in the third year with the recruitment protocol in place.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2004
- Accession Number
- ADA425986
Entities
People
- Radoslav Goldman
Organizations
- Georgetown University